How inForce improved fibromyalgia and auto immune chronic pancreatitis

Kenny, As you know I started with InLife when they first kicked off the smokeless cigarettes! I am a distributor and have been taking InLife for about 10 months and feel like me again and have more energy as well!! I have fibromyalgia and auto immune chronic pancreatitis! Within two weeks my fibromylagia pain went from a 10 to a 1 and most of the time NO pain at all! I was still dealing with my chronic pancreatitis. Well for the past 3 months I have no pain from my pancreas thanks to InForce. It may have taken sometime for the pancreas…..BUT the best besides NO pain is this; When your Amylase and Lipase levels are above the normal range you are having pancreas attack so here are the normal ranges and what mine were and are now!!!

Amylase-normal range 28-100

Before InForce my range was. 290-320

Now. 91

Lipase-normal range 16-63

Before InForce my range 160-210

Now. 57

I take 3 InForce in morning half hour on empty stomach And 3 InForce in evening half hour on empty stomach all before meals! I’m a walking testimonial of how much I believe in what InForce can do! I would love to share this with anyone who is who may have same conditions as myself or anyone who is considering whether or not to take InForce!

Cindy Frost Sent from my Verizon Wireless BlackBerry

Anticancer effects and mechanisms of polysaccharide?K (PSK): implications of cancer immunotherapy.

Fisher M, Yang LX.

Radiobiology Laboratory, St. Mary’s Medical Center, California Pacific Medical Center Research Institute, San Francisco

94118, USA.

Abstract

Polysaccharide-K (polysaccharide-Kureha; PSK), also known as krestin, is a unique protein-bound polysaccharide, which

has been used as a chemoimmunotherapy agent in the treatment of cancer in Asia for over 30 years. PSK and

Polysaccharopeptide (PSP) are both protein-bound polysaccharides which are derived from the CM-101 and COV-1 strains

of the fungus Coriolus versicolor by Japanese and Chinese researchers, respectively. Both polysaccharide preparations

have documented anticancer activity in vitro, in vivo and in human clinical trials, though PSK has been researched longer

and has therefore undergone more thorough laboratory, animal and clinical testing. Several randomized clinical trials have

demonstrated that PSK has great potential as an adjuvant cancer therapy agent, with positive results seen in the adjuvant

treatment of gastric, esophageal, colorectal, breast and lung cancers. These studies have suggested the efficacy of PSK as

an immunotherapy or biological response modifier (BRM). BRMs potentially have the ability to improve the “host versus

tumor response,” thereby increasing the ability of the host to defend itself from tumor progression. The mechanisms of

biological response modification by PSK have yet to be clearly and completely elucidated. Some studies suggest that PSK

may act to increase leukocyte activation and response through up-regulation of key cytokines. Indeed, natural killer (NK)

and lymphocyte-activated killer (LAK) cell activation has been demonstrated in vivo and in vitro, and recent genetic studies

reveal increased expression of key immune cytokines in response to treatment with PSK. An antimetastatic action of PSK

has also been demonstrated and is perhaps attributed to its potential to inhibit metalloproteinases and other enzymes

involved in metastatic activity. PSK has also been shown to cause differentiation of leukemic cells in vitro, and this effect

has been attributed to induction of differentiation cytokines. PSK has further been shown to have antioxidant capacity which

may allow it to play a role as a normal tissue chemo- and radio-protector when used in combination with adjuvant or

definitive chemotherapy and/or radiotherapy in the treatment of cancer, while it may also enable it to defend the host from

oxidative stress. Interestingly, studies have also shown that PSK may actually inhibit carcinogenesis by inhibiting the action

of various carcinogens on vulnerable cell lines. This action of PSK may play a role in preventing second primary tumors

when an inducing agent, such as tobacco or asbestos, is suspected and may also prevent second malignancies due to the

carcinogenic effects of radiotherapy and cytotoxic chemotherapy. Another very important aspect of chemoimmunotherapy,

in general is that it may be used on debilitated patients such as those with AIDS and the elderly who might otherwise be

denied potentially helpful adjuvant cytotoxic chemotherapy. Further determination of the mechanisms of these anti-cancer,

immunostimulating and biological response modifying effects of PSK as well as of other protein-bound polysaccharides is

certainly warranted. Indeed, with modern cellular and molecular biology techniques, a better understanding of the specific

Molecular effects of PSK on tumor cells as well as leukocytes may be determined. Much of the research that has been done

on PSK is outlined in this paper and may serve as a foundation toward determining the mechanisms of action of this and

other protein-bound polysaccharides in the treatment of cancer. This information may open new doors in the development

of novel strategies for the treatment of malignancies using adjuvant immunotherapy in combination with surgery,

chemotherapy and/or radiotherapy.

PMID: 12168863 [PubMed – indexed for MEDLINE]

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The culture duration affects the immunomodulatory and anticancer effect of polysaccharopeptide derived from Coriolus versicolor

Cheuk-Lun Lee, Xiaotong Yang, Jennifer Man-Fan Wan

Department of Zoology, Kardoorie Biological Science Building, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, China

Received 24 December 2003; accepted 5 October 2004

Abstract

Polysaccharopeptide (PSP) derives from the medicinal mushroom Coriolus versicolor is considered a biological response modifier with potential pharmaceutical applications. Significant literatures support the immune and anticancer functions of PSP; however, standardization is of big concern because variable biotechnological factors can affect both the chemical and biological properties of PSP. In this study, the extracts of PSP obtained at different days from the Coriolus versicolor culture were tested in vitro for their immune function on human normal peripheral blood mononuclear cells (PBMC) and cytotoxicity on the human leukemia Molt 4 cells. Over the 10-days culture period, both biomass and peptide/polysaccharide content were increased with time. The increase in proliferation index of PBMC and their production of interleukin 1 beta (IL-1_), tumor necrosis factor alpha (TNF-_) and gamma interferon (IFN-_) in the presence of PHA strengthens the correlation between culture duration and biological potency of PSP. The growth inhibition of the Molt 4 cells by PSP also depended on its maturity. Flow cytometry analysis on cell cycle and cell death (apoptosis) of Molt 4 cells indicated that the anticancer mechanism of PSP is related to its ability to induce S-phase cell arrest and apoptosis, respectively. Together, these results suggest that monitor the harvest duration is critical for the quality control of polysaccharopeptide in the biotechnological industry.

© 2005 Elsevier Inc. All rights reserved. Keywords: Coriolus versicolor; Polysaccharopeptide; Flow cytometry; High performance liquid chromatography; Peripheral blood mononuclear cells; Molt 4

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[Randomized controlled study on adjuvant immunochemotherapy with PSK in curatively resected colorectal cancer. The Cooperative Study Group of Surgical Adjuvant Immunochemotherapy for Cancer of Colon and Rectum]

[Article in Japanese]

Mitomi T, Tsuchiya S, Iijima N, Aso K, Suzuki K, Nishiyama K, Amano T, Takahashi T, Murayama N, Oka H, et al.

Dept. of Surgery II, Tokai University.

Abstract

To evaluate of adjuvant immunochemotherapy with PSK in curatively resected colorectal cancer, randomized controlled

study by 35 institutions in Kanagawa prefecture was conducted. From March 1985 till February 1987, 462 patients were

assigned one of two different regimens. 448 patients (97.0%) of them satisfied the eligibility criteria. Control group

received mitomycin C intravenously on the day and the day after the operations respectively followed by 5-FU orally over

for 6 months. PSK group received in addition to mitomycin C and 5-FU as in control group, PSK orally for over 3 years. By

February 1989, follow up studies of the patients after their operations had been carried out for two years to four years.

The disease free curve and the survival curve of PSK group were higher than those of control group, differences between

the two groups were statistically significant (Disease free curve: P = 0.0096, survival curve: p = 0.0391). From these

results, adjuvant immunochemotherapy with PSK was considered beneficial for curatively resected colorectal cancer.

PMID: 2500070 [PubMed – indexed for MEDLINE]

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The Electrophretical Analysis of the Components of Polysaccharide-peptide (PSP)

Xiao-tong Yang and Yong Zhuang Shanghai Teachers University

Abstract

The components of polysaccharide-peptide (PSP) are analysed by electrophoresis of cellulose acetate membrance and of polyacrylamide gel. The result shows that there are 2 kinds of glycoprotein and 2 kinds of polysaccharide. There are obvious differences between the numbers of components and the nature of electrophoresis of PSP and the polysaccharides of the fruit-bodies of Yun Zhi (CVP).

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Usefulness of immunomodulators for maturation of dendritic cells.

Ogihara T, Iinuma H, Okinaga K.

Department of Surgery, Teikyo University School of Medicine, Tokyo 173-0003, Japan.

Abstract

Biological response modifiers (BRMs) augment the cytotoxic activity of various effector cells by the induction of multiple

cytokines and suppression of immunosuppressive factors. BRMs are used extensively in adjuvant therapy for gastric

cancer in Japan. In dendritic cell (DC)-based vaccine therapy, the quality of DCs is important in inducing strong antitumor

immunity. A good manufacturing practice (GMP) grade agent for DCs maturation is desirable for safety. Here we report

the effects of two BRMs, OK432 and PSK, which are GMP grade agents for the functional maturation of DCs. OK432 and

PSK were examined in vitro, and compared with lipopolysaccharide (LPS) and a cytokine cocktail (IL-1beta, TNF-alpha,

IL-6 and PGE2). In the immunophenotypical analysis, the expression of CD80 and CD83 of DCs stimulated with OK-432

increased significantly compared with PSK and medium, and this up-regulation was the same as levels of DCs stimulated

with cytokine cocktail. DCs stimulated with OK-432 showed significantly higher production of IL-12 and Th1-type cytokines

(IL-2 and IFN-gamma) compared with DCs stimulated with LPS or cytokine cocktail. OK-432 stimulated DCs could induce

the significantly high level of cytotoxic T cell activity compared with PSK-stimulated or unstimulated DCs. These results

suggest that OK432 is a GMP-grade reagent that promotes functional maturation of DCs and could be applied in

DC-based vaccinations.

PMID: 15254744 [PubMed – indexed for MEDLINE]

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Stimulation of interferon-gamma-induced human myelogenous leukemic cell differentiation by high molecular weight PSK subfraction.

Display Settings: Abstract

Anticancer Res. 1990 Jan-Feb;10(1):55-8.

Kim F, Sakagami H, Tanuma S, Konno K.

First Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.

Abstract

PSK, a protein-bound polysaccharide extracted from the mycelia of Coriolus versicolor (Fr.) Quel, stimulated tumor

necrosis factor-induced cytotoxicity against mouse L-929 fibroblast. PSK also stimulated interferon-gamma-induced

differentiation of human myelogenous leukemic U-937 and THP-1 cells. The differentiated cells had higher proportions of

cells that expressed NBT-reducing activity and alpha-naphthyl acetate esterase activity. Among four PSK subfractions, the

highest molecular weight fraction (MW greater than 200 kD) had the most potent stimulating activity. This is the first report

regarding direct PSK modulation of cytokine action.

PMID: 2110432 [PubMed – indexed for MEDLINE]

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Usefulness of immunomodulators for maturation of dendritic cells.

Display Settings: Abstract

Int J Oncol. 2004 Aug;25(2):453-9.

Ogihara T, Iinuma H, Okinaga K.

Department of Surgery, Teikyo University School of Medicine, Tokyo 173-0003, Japan.

Abstract

Biological response modifiers (BRMs) augment the cytotoxic activity of various effector cells by the induction of multiple

cytokines and suppression of immunosuppressive factors. BRMs are used extensively in adjuvant therapy for gastric

cancer in Japan. In dendritic cell (DC)-based vaccine therapy, the quality of DCs is important in inducing strong antitumor

immunity. A good manufacturing practice (GMP) grade agent for DCs maturation is desirable for safety. Here we report

the effects of two BRMs, OK432 and PSK, which are GMP grade agents for the functional maturation of DCs. OK432 and

PSK were examined in vitro, and compared with lipopolysaccharide (LPS) and a cytokine cocktail (IL-1beta, TNF-alpha,

IL-6 and PGE2). In the immunophenotypical analysis, the expression of CD80 and CD83 of DCs stimulated with OK-432

increased significantly compared with PSK and medium, and this up-regulation was the same as levels of DCs stimulated

with cytokine cocktail. DCs stimulated with OK-432 showed significantly higher production of IL-12 and Th1-type cytokines

(IL-2 and IFN-gamma) compared with DCs stimulated with LPS or cytokine cocktail. OK-432 stimulated DCs could induce

the significantly high level of cytotoxic T cell activity compared with PSK-stimulated or unstimulated DCs. These results

suggest that OK432 is a GMP-grade reagent that promotes functional maturation of DCs and could be applied in

DC-based vaccinations.

PMID: 15254744 [PubMed – indexed for MEDLINE]

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Study on Anti-tumor Action of PSP

R.T. Chen, A.M. Zhou, B. Xu Department of Pharmacology I Shanghai Institute of Materia Medica, Academia Sinica

Abstract

It has been reported that some polysaccharides possess antitumor action. PSP is a glycopeptide isolated from Coriolus versicolor by Yang et al. Its physiological properties have been investigated. In the present work we studied the antitumor action of PSP in vitro experiments.

Summary

PSP at the doses of 500 or 1000ug/ml produced inhibitory effect on P388 luekemia cells by 79-96%. At the dose of 1000 or 2000ug/ml PSP caused the inhibition of [3H]UR or [3H]TdR incorporation into RNA and DNA in Ehrlich ascites carcinoma cells was found to be the inhibition rate 50-80% or 27-47% respectively.

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The Influence of PSP on the Behavior and Growth of the Fetus in Rats.

Zheng-de Zhang1, Zhen-bin Qian2, Lan-feng Zhou2 and Bin Xu1 1 Shanghai Institute of Materia Medica, Academia Sinica 2 Department of Toxicology, Shanghai Institute of Labour Health and Occupational Diseases

Abstract

PSP is a new immunomodulating agent prepared and developed by Professor Qing-yao Yang. In the present work its influence on the behavior and growth of fetus in 1st and 2nd generation of rats was investigated.

For behavior study the squirrel wheel test, rotating cylinder test and passive avoidance test were performed, PSP was administered orally at 60, 600 and 6000mg/kg. The results showed that no marked difference in the change of behavior was found between the control and treatment groups. The brain weights of two-generation rats had no marked change either.

For teratogeny test 110 rats were involved PSP was administered orally at 60, 600 and 6000mg/kg in different groups for 10 days. The conditions of the maternal body, embryonic survival and the function and growth of the fetuses were examined. After statistical tests no marked difference was observed in the control and treatment groups.

The data observed showed that PSP has no noticeable toxicity on fetus growth and on the behavioral performance of 1st and 2nd generation rats

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