Reversal of inhibition of reactive oxygen species on respiratory burst of macrophages by polysaccharide from Coriolus versicolor.

Jun L, Mei Z, Yuan C.

Department of Biochemistry, First Medical College of PLA, Guang Zhou, P.R.C.


Using a luminol-dependent, chemiluminescence assay we found tert-butylhydroperoxide to be a strong inhibitor of the respiratory burst of mouse peritoneal macrophages. However, the inhibition of respiratory burst induced by tert-butylhydroperoxide could be prevented after the interperitoneal injection of polysaccharide from Coriolus versicolor (PSK). Further investigation showed that glutathione peroxidase activity was markedly elevated in PSK-treated macrophages. After incubation with tert-butylhydroperoxide, higher activity of glutathione peroxidase was maintained in PSK-treated macrophages. These results suggest that the immunological function of macrophages is related to the activity of glutathione peroxidase. The non-specific immunopolysaccharide might protect macrophages from the damage induced by reactive oxygen species by enhancing antioxidative capacity.

PMID: 8505152 [PubMed – indexed for MEDLINE]